Purification and characterization of potato lectin.

A New Galactose-Specific Lectin from Clerodendrum infortunatum

Background: The ethno-medical significance of Clerodendrum genus raises the interest towards the characterization of its seed lectin by inexpensive and most effective technique.Objective: The focus of this study is the purification, characterization, and evaluation of the antioxidant and antiproliferative potential of a galactose-specific lectin from Cler...

Purification of a novel chitin-binding lectin with antimicrobial and antibiofilm activities from a bangladeshi cultivar of potato (Solanum tuberosum).

A new chitin-binding lectin was purified from a Bangladeshi cultivar 'Deshi' of potato (Solanum tuberosum L.) through anion-exchange and affinity chromatographies using a chitin column. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed the molecular mass of the lectin as 20,000 Daltons. This molecular mass was almost half of the molecular masses of chitin-binding lect...

Purification and Characterization of a Mucin Specific Mycelial Lectin from Aspergillus gorakhpurensis: Application for Mitogenic and Antimicrobial Activity

BACKGROUND Lectins are carbohydrate binding proteins or glycoproteins that bind reversibly to specific carbohydrates present on the apposing cells, which are responsible for their ability to agglutinate red blood cells, lymphocytes, fibroblasts, etc. Interest in lectins has been intensified due to their carbohydrate specificity as they can be valuable reagents for the investigation of cell surf...

Purification, characterization and antifungal activity of LPA a lectin from Pachira aquatica seeds: their possible role in plant defence

Background The lectins are an important class of proteins ubiquitous in nature, which plays different roles and functions in biological processes such as recognition molecules within the immune system in animals and as storage proteins or in defence mechanisms against pest and pathogens in plants. Oliveira et al. [1] showed that rats fed on a lectin-rich diet from Pachira aquatica seeds had a p...

The purification and properties of the lectin from potato tubers, a hydroxyproline-containing glycoprotein.

1. Potato lectin has been purified and shown to be a glycoprotein containing about 50% of carbohydrate. Most of the sugar residues (92%) are arabinose; small amounts of galactose, glucose and glucosamine are also present. 2. The most abundant amino acid is hydroxyproline (16% of the residues), 11.5% of the residues are half-cystine and phenylalanine is absent. The lectin also contains about one...

Rapid affinity-purification and physico- chemical characterization of pumpkin (Cucurbita maxima) phloem exudate lectin